Sequence capture of WW2: H7 - SRA

SRX24544693: Sequence capture of WW2: H7
1 ILLUMINA (Illumina MiSeq) run: 1.2M spots, 743M bases, 407.6Mb downloads

Design: We extracted DNA from avian blood using a standard ethanol based extraction protocol. We tested for haemosporidian DNA with nested PCR and sequenced haemosporidian cytochrome b with Sanger sequencing to confirm haemosporidian species and lineage. We captured targeted regions of the extracted DNA using a myBaits Hybridization Capture for Targeted NGS Kit with the Swift Biosciences Accel-NGS 2S Hyb DNA Library Kit and KAPA HiFi HotStart ReadyMix PCR Kit. To do this, we sheared the 48 DNA samples using an M220 Focused-ultrasonicator (Covaris, MA, USA) and then built indexed Illumina libraries from all samples. We next made six pools, each made up of eight libraries at equimolar concentrations, and we performed the sequence capture on each pool separately. We sequenced the captured DNA on an Illumina MiSeq.

Submitted by: University of Delaware

Study: Sequence capture of Haemoproteus majoris lineage WW2

PRJNA1111424 • SRP507542 • All experiments • All runs

show Abstracthide Abstract

Sequence genes of the avian haemosporidian parasite Haemoproteus majoris (lineage WW2) from birds in southern Sweden to assess population genetic structure of the parasite.

Sample:

SAMN41391303 • SRS21289166 • All experiments • All runs

Organism: Haemoproteus majoris

Library:

Name: lib7

Instrument: Illumina MiSeq

Strategy: Targeted-Capture

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 1.2M spots, 743M bases, 407.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR29019002	1,234,203	743M	407.6Mb	2024-05-14

ID:: 32862551

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity